#Using an adaptor on serial cloner how to#
#Using an adaptor on serial cloner full#
#Using an adaptor on serial cloner windows#

#Using an adaptor on serial cloner full#

A bug affecting protruding end sequence in the construct window when using a full antiparallel liner insert A bug in the Find window sometime preventing the detection of multiple RE hits A display problem in RE List window (hidden sites) A display problem in RE List window (Blunt sites) A bug in coloring the text obtained after a 'Copy as Formatted' command Improved the behavior of choose site button in the Graphic map Improved the behavior of the RE List window

A problem setting up character size in preferences for the RE map A parsing problem when a Feature list was empty

#Using an adaptor on serial cloner windows#

Problem of highlight when searching for sequence under windows (Find/Sequence window) Display of very big DNA ladder (Virtual cut) Features with additional 5' or 3' sequences are now also recognized in the anti-sense direction. Graphic display of type IIs restriction sites. Header of Graphic map now wrap properly Avoid duplicating Features wending PCR, Ligation, etc. Avoid repetition of annotation detection when scanning Use the top-most opened sequence windows as default names in Align Better conservation of Features when extracting a fragment

In the Find Window : Frame 0 is now labelled '3' Change character size of Graphic Map header depending on sequence name size Improve recognition of att site in the Gateway construction window Improved parsing of Serial Cloner GenBank format Improved text contrasting in dark background (REsites, Graphic Map) Colorize annotation when importing from non-serial cloner format Check all/uncheck all command in RE List ALT-Click on a Graphic Map Window brings up the parent Sequence Window Rapid deletion of a Feature in the list (Seq Window) using ALT-DELETE or CTR-SHIFT-DELETE Recover PCR windows after (hopefully rare) crashing A button to show/hide unchecked RE sites in RE List window Allows to change size of the list Field in the Feature Tab Automatic recognition of pasted NCBI and EMBL degenerate sequences Parse (Import) Degenerate NCBI and EMBL GenBank format A preference to choose or not to Box and color RE Names in Graphic map (Unique sites) A preference to choose to box or not RE-site/Features names (Graphic Map) A preference to modify RE name character size (Graphic Map) A preference to modify Feature character size (Graphic Map) Incomplete ORF are now detected and shown in italic in the find window A preference to show the AA letter aligned with the middle of the codon (RE Map) 'Remove duplicated Features' menu entry Transform a DNA sequence window into a degenerate DNA sequence and vice versa (menu and contextual menu) Preference setting to change Read out speed

Direct import of DNAStar EditSeq files (.seq) Direct import of VectorNTI single and multisequence files (.ma4) (see provided example file) Direct import of MacVector files (.nucl) Generate sense and antisense strand after C>T bisulfite conversion 'Import Codon Usage' window to import tables directly from the web A preference option to choose the Codon usage table to be used for reverse translation Reverse translation in the protein menu BLAST protein Sequence now in the protein menu. Serial Cloner 2.5 now allows to manipulate protein sequences, do reverse-translation, and import codon frequency tables from the internet. It is also posible to send directly BLAST request at the NCBI and obtain the result inside a Web interface.

#Using an adaptor on serial cloner how to#

You will also find a Restriction Enzyme library management interface, Additional tools, like a web browser for direct import of NCBI and EMBL entries, a virtual cutter to prepare restriction analysis or a silent restriction map generator to find how to introduce restriction sites without modifying the translated peptide are also provided. Features are now visible when aligning locally. Finally, Serial Cloner provides an interface to align two sequences using a local algorithm or the BLAST2Seq NCBI server. An additional interface allows easy Gateway(tm) cloning for both BP and LR reactions. Just select, blunt if you need, and click the Ligate button. Finally, you can assemble fragments, obtained by PCR, adaptor/shRNA synthesis or simply by graphically selecting fragments between restriction sites. shRNA constructions based on pre-defined scaffolds are also automated. PCR-based fragment or synthetic adaptors.